Friday , 14 May 2021

Establishment of efficient protocol in-vitro Shoot Regeneration from Nodal Explants. Withania somnifera (L.) Dunal

Jaya Sharma*
Department of Biotechnology and Microbiology, Unique College Jawahar Chowk.TT Nagar Bhopal, M.P., India 

The Present investigation has given a method for in vitro micropropagation of a valued medicinal plant Withania somnifera. In this study we used nodal part of Withania somnifera as explants. Explants were cultured on MS media (Murashige and Skoog) supplemented with different concentrations of Growth hormones like BAP (0.1-2.0mg/l) NAA (0.1-1.0mg/l). The excellent shoot induction followed by 1mg/l BAP+ 0.5mg/l NAA in this combination was observed as 90% and maximum shoot length was recorded (4.08cm.). Regenerated shoots were rooted on MS medium supplemented with IBA (0.1-1.0) mg/l and NAA (0.1-1.0) mg/l. maximum root length ( contributed by IBA at 1.0 mg/l on MS medium.
Key words: In vitro, micropropagation, Withania somnifera, medicinal plant.

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