Sunday , 23 January 2022

Development of new simultaneous RP-HPLC method for the estimation of Glecapravir and Pibrentasvir in tablet dosage form

M. Lakshmi Prasanna*, C.S. Parameswari, B.V. Ramana, G. Nagarajan
Dr. K.V. Subba Reddy Institute of Pharmacy, Kurnool, Andhra Pradesh, India

The aim present research work to development and validation of RP-HPLC method for the simultaneous estimation of Glecapravir and Piberentasvir. Chromatographic separation was evaluated by Xterra C18 column (250 X 4.6 mm, 5 µm) using the mobile phase consisting of 50% Water: 50% Acetonitrile and the mobile phase was pumped at a flow rate of 1.0 mL/min and detection was done by UV detector at 255 nm. The retention time of Glecapravir and Piberentasvir were found to be 2.205 min and 4.996 min. The linearity was obtained in the range of 100-500µg/ml for Glecapravir and 40-200 µg/ml for Piberentasvir with correlation coefficient was 0.999. The proposed method was found to be simple, accurate, precise, robust and cost effective. It can be applied for routine quality control analysis for simultaneous estimation of Glecapravir and Piberentasvir in pharmaceutical dosage forms.
Keywords: Glecapravir, Piberentasvir, RP-HPLC, Mobile phase, Acetonitrile, Retention time.

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