Monday , 17 January 2022

Development of New Simultaneous RP-HPLC Method for the Estimation of Dolutegravir and Rilpivirin in Tablet Dosage Form

V. Yugandhar*, B.V. Ramana, G. Nagarajan
Dr. K.V. Subba Reddy Institute of Pharmacy, Kurnool, Andhra Pradesh, India

The aim present research work to development and validation of RP-HPLC method for the simultaneous estimation of Dolutegravir and Rilpivirin. Chromatographic separation was carried on Inertsil ODS C18 column (200 X 4.6 mm, 5µm) using the mobile phase consisting of 0.1% OPA:  Acetonitrile (80: 20). The mobile phase was pumped at a flow rate of 1.5 mL/min and detection was done by UV detector at 230 nm. The retention time of Dolutegravir and Rilpivirin were found to be 3.417min and 4.392 min. The linearity was obtained in the range of 50-250 µg/ml for Dolutegravir and 25-125 µg/ml for Rilpivirin with correlation coefficient was 0.999. The proposed method was found to be simple, accurate, precise, robust and cost effective. It can be applied for routine quality control analysis for simultaneous estimation of Dolutegravir and Rilpivirin in pharmaceutical dosage forms.
Keywords: Dolutegravir, Rilpivirin, RP-HPLC, Mobile phase, Acetonitrile, Retention time

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