Monday , 21 September 2020

Development and Validation of RP-HPLC Method for the Estimation of Olmesartan Medoxomil and Chlorethalidone in Tablet Dossage Form

Nidamanuri Vamsi Krishna*, V. Hari Baskar, M. Gobinath, Ramesh Dhani, V. Pavan Kumar, D. Madhu
Department of Pharmaceutical Analysis, Ratnam Institute of Pharmacy, Pidathapolur, Muthukur, SPSR Nellore, A.P, India

A simple, Accurate, precise method was developed for the simultaneous estimation of the olmesartan medoxomil and chlorethalidone in Tablet dosage form. Chromatogram was run through Kromasil (250mm 4.6mm, 5µ). Mobile phase containing Buffer and Acetonitrile in the ratio of 55:45A was pumped through column at a flow rate of 1ml/min. Temperature was maintained at 30°C. Optimized wavelength for Olmesartan medoxomil and Chlorethalidone was 240nm. Retention time of Olmesartan medoxomil and Chlorethalidone were found to be 4.18 min and 3.30 min. %RSD of the Olmesartan medoxomil and Chlorethalidone were and found to be 0.3 and 1.6 respectively. %Recover was Obtained as 100.04% and 100.0% for Olmesartan medoxomil and Chlorethalidone. LOD, LOQ values were obtained from regression equations of Olmesartan medoxomil and Chlorethalidone were 0.03ppm, 0.09ppm and 0.04ppm, 0.11ppm respectively. Regression equation of Olmesartan medoxomil is y = 35709x + 752.6, and of Chlorethalidone is y = 27028x + 420.7.
Keywords: Olmesartan medoxomil, Chlorethalidone, RP-HPLC

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