Analytical Method Development and Validation by RP-HPLC for Simultaneous Estimation of Cefixime and Ornidazole in Combined Tablet Dosage Form

M. Shirisha*, Ramesh Dhani, V. Haribaskar, M. Gobinath, V. Amaravathi
Department of Pharmaceutical Analysis, Ratnam Institute of Pharmacy, Pidathapolur, Muthukur, SPSR Nellore, A.P, India

A B S T R A C T
A simple, Accurate, precise method was developed for the simultaneous estimation of the Cefixime and Ornidazole in Tablet dosage form. Chromatogram was run through ODS (250mm 4.6mm, 5µ). Mobile phase containing Buffer and Acetonitrie and methanol in the ratio of 45:45:10A was pumped through column at a flow rate of 1ml/min. Buffer used in this method was 0.01N KH₂PO₄ buffer at pH 4.6. Temperature was maintained at 30°C. Optimized wavelength for Cefixime and Ornidazole was 220nm. Retention time of Cefixime and Ornidazole were found to be 4.0 min and 2.8 min. %RSD of the Cefixime and Ornidazole were and found to be 0.66 and 0.61 respectively. %Recover was obtained as101.04% and 100.41% for Cefixime and Ornidazole respectively. LOD, LOQ values are obtained from regression equations of Cefixime and Ornidazole were 0.74ppm, 0.72ppm and 2.23ppm, 2.19ppm respectively. Regression equation of Cefixime is y = 2829.x + 1707, and of Ornidazole is y = 1935x + 866.3.
Keywords: Cefixime, Ornidazole, RP-HPLC.