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SK. Kowsar*, V. Hari Baskar, M. Gobinath, Ramesh Dhani, V. Pavan Kumar, K. Giridhar Reddy
Department of Pharmaceutical Analysis, Ratnam Institute of Pharmacy, Pidathapolur, Muthukur (M), Nellore, A.P, India.
A B S T R A C T
A simple, accurate and reproducible RP-HPLC method has been developed for simultaneous estimation of Abacavir and Lamivudine in bulk and tablet dosage form. Which are widely used as anti HIV drugs. Chromatography was carried out the column inertsil ODS C18 (250×4.6mm, 5µm) using a mobile phase composition of Methanol and Potassium dihydrogen Phosphate buffer pH-4.0 (60:40) at flow rate of 1.0ml/min. The detection was made at 257nm.The retention times of Abacavir and Lamivudine are 3.0min and 3.9min respectively. The method was validated for system suitability, precision, linearity, accuracy, robustness, LOD and LOQ. The drug obeys linearity within the concentration range of 15-90µg/ml for Abacavir and 7.5-45µg/ml for Lmivudine .The proposed method was validated as per ICH guide lines and it was found to be Suitable for the routine quality control analysis of the bulk and in its tablet dosage forms.
Keywords: Abacavir, Lamivudine, RP-HPLC, Methanol, Anti retroviral agents.
