Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida, Sec-125, Noida-201303 (UP), India
A B S T R A C T
Amylase is starch-degrading enzyme and exploited in textile, leather, wood, paper and detergent industries for desizing and washing of fabrics. Previously, it has been immobilized on to various compatible supports by several techniques to increase its thermal and storage stability. Its immobilization onto bovine serum albumin (BSA) was found to be very significant to prepare its BSA loaded nano-particles. In this study, Pearl millet amylase loaded almond oil driven BSA nanoparticles (BSANPs) were used and made bio-active with 35U of alkaline protease for their biodegradation. Then, bio-active enzyme-BSA bound nanoparticles was used as nano-active detergent additives with chosen wool shampoo named, Pure Nature WOLL-Shampoo to test their stain removal efficacy from stained woolen fabric without affecting the delicacy and softness of the chosen fabric. In this study, chosen stains were comprised of daily routine stains of boot polish and hair color dye cream named, Cherry Blossom Black and Garnier Nutrisse Black respectively. Sometimes, these stains are very difficult to wash off from the woolen fabric/cashmere/ merino/ mohair fabrics and require couple of pre-washing practices. So, keeping this domestic or industrial washing issue, selected stains are chosen very thoughtfully. Hence, from this study, an effective washing data was carried out to wash off the selected stains form woolen fabric when washed with chosen wool shampoo along with bio-active prepared amylase bound BSANPs solution as compared their washing with chosen wool shampoo alone.
Keywords: Cherry Blossom Black; Garnier Nutrisse Black; BSANPs; Bovine serum albumin nanoparticles; Almond oil; Wool shampoo.