Monday , 24 April 2017

Virucidal potential of saponin extricated from Emblica officinalis and Ficus religiosa

Amit Gupta1 and Sushama R Chaphalkar2
1Department of Immunology, Vidya Pratishthan’s School of Biotechnology, Baramati, Maharashtra, India
2Director, Vidya Pratishthan’s School of Biotechnology, Baramati, Maharashtra, India

A B S T R A C T
This study was conducted in order to determine the importance of crude saponins from the leaves of various medicinal plants viz. Emblica officinalis and Ficus religiosa for the treatment of various infectious diseases particularly intracellular or extracellular pathogens. However, the role of saponin is less explored especially as an virucidal agent. The objective of our proposed study is to determine its virucidal activity of crude saponin extracted from Emblica officinalis and Ficus religiosa against New castle disease virus (NDV) which is determined in animal (Swiss mice) model studies. Swiss mice (n = 5) were immunized subcutaneously on day 0 and day 7 with variable doses of saponins (50, 100 and 200 µg) along with NDV (1:100 dilution) and determined its proliferation assay, CD4/CD8 estimation and Th1 (IFN-gamma and TNF alpha) type of cytokines from cell culture supernatant by Elisa. The results showed that saponin (200 µg) at higher doses showed drastically decreased in proliferation, CD4/CD8 estimation and Th1 (IFN-gamma and TNF alpha) type of cytokines from cell culture supernatant. NDV (1:100 dilution) used as standard for these studies and the results showed that enhancement of lymphocyte proliferation, CD4/CD8 estimation and Th1 (IFN-gamma and TNF alpha) type of cytokines from cell culture supernatant. Overall, the results showed that saponin from Emblica officinalis and Ficus religiosa showed virucidal activity.
Keywords: Emblica officinalis; Ficus religiosa; saponin; virucidal

Comments are closed.

Scroll To Top