Screening of In-Vitro Antioxidant Activity of Poly Herbal Methanolic Extract

Ganta Radhika Reddy*, Dr. P Mani Chandrika, K. Shailaja, K. Priyanka, D. Ratna Kumari, B. Sandhya, T. Sarika, P. Shalini
Bojjam Narasimhulu Pharmacy College for Women, Vinay Nagar, Saidabad, Hyderabad, Telangana, India.

A B S T R A C T
The present study was carried out to evaluate antioxidant activity of poly herbal methanolic extract of peels of Beta vulgaris (beetroot), Luffinae aegyptiaca (ridge gourd), Cucumis sativus (cucumber), and flower buds of Syzygium aromaticum (clove). The plant parts i.e the fruits were collected from the local market and the peels were removed, they were shade dried and grinded to get the coarse powdered material. The coarse powder was subjected for extraction in soxhlet extraction apparatus using methanol as solvent and concentrated using Rota vapour. The product was subjected to lyophillization process to get fine powder. The qualitative screening of the poly herbal extract revealed the presence of alkaloids, glycosides, saponins, phenolics and flavonoids. The collected fine powder was used to evaluate the in-vitro antioxidant activity by DPPH free radical scavenging method and Hydrogen peroxide radical scavenging (H₂O₂) assay. IC₅₀ Value of standard ascorbic acid was found to be 190 and poly herbal extract was found to be 197 by DPPH free radical scavenging method. IC₅₀ Value of standard ascorbic acid was found to be 190 and poly herbal extract was found to be 193 by Hydrogen peroxide radical scavenging (H₂O₂) assay. The IC₅₀ value of the poly herbal extract was found to be near to the standard ascorbic acid value.
Keywords: Antioxidant activity, Ascorbic acid, .Beta vulgaris, Cucumis sativus, Luffinae aegyptia, Syzygium aromaticum.