Guvvala Triveni, G. Dharmamoorthy*
Department of Pharmaceutical Analysis, Krishna Teja Pharmacy College, Tirupati-517506, Andhra Pradesh, India
A simple, Accurate, precise method was developed for the simultaneous estimation of the L-methyl folate and Escitalopram in bulk and its pharmaceutical dosage form.Chromatographic separation was carried on Denali C18(150 x 4.6 mm, 5µ) column . Mobile phase consist of Phosphate buffer and Acetonitrile taken in the ratio of 50:50was pumped through column at a flow rate of 1.0 ml/min. Mobile phase PH 6.8 was adjusted with 0.01N KH2PO4. Optimized wavelength selected was 212 nm. Retention time of L-methyl folate and Escitalopram were found to be 2.188min and 3.822min. %RSD of the L-methyl folate and Escitalopram were and found to be 0.5 and 0.6 respectively. %Recovery was obtained as 98.82% and 100.16% for L-methyl folate and Escitalopram respectively. LOD, LOQ values obtained from regression equations of L-methyl folate and Escitalopram were 0.07, 0.22 and 0.84, 2.55 respectively. Regression equation of L-methyl folate is y y = 9276.x + 180.2, and y = 9771.x + 4371.. of Escitalopram. The developed method was simple and economical that can be adopted in regular Quality control test in Industries.
Keywords: L-methyl folate, Escitalopram, RP-HPLC