Dr. K. Surendra*, M.V. Jeevanandham, E. Srikanth, Narayana
Rao’s College of Pharmacy, Chemudugunta, Venkatachalam, Nellore, Andhra Pradesh-524320
A B S T R A C T
High performance LC-NMR is an advanced analytical technique for the identification of components in pharmaceutical and biological mixtures without the need of chemical separation. The advantages of this technique NMR directly coupling with HPLC instrumentation must be weighed against compromises in performance made to each technique to achieve a hyphenated system. The coupling of LC separation with NMR characterization has been employed in a wide range of applications. The implementation of mass spectrometry in LC–NMR is also useful on account of the molecular weight and fragmentation information that it provides, especially when new plant species are studied. The present review describes that history and development of LC—NMR, Instrumentation along with applications. LC-NMR was also coupled with other techniques such as two-dimensional NMR measurements (LC-2D NMR), Mass spectroscopy or MS (LC-NMR/MS) and solid phase extraction or SPE (LC-SPE-NMR) to give rise to new range of separation and characterization of applications have a high sensitivity.
Keywords: LC-NMR, HPLC, Chemical separation, Instrumentation, LC-2D NMR, LC-NMR/MS, LC-SPE-NMR