Thursday , 23 March 2017

Quantitative investigation of phytochemicals in Nakshtra plants and distribution analysis of ketones pertaining to LC-MS

Ajam C Shaikh, Amit Gupta and Sushama R Chaphalkar*
Vidya Pratishthan’s School of Biotechnology (VSBT, Research Centre affiliated to Savitribai Phule Pune University), Baramati, Maharashtra, India

Majority of population in developed and under developed countries totally relies on traditional forms of medicine, mainly plant based to meet the primary health care needs of the people. These medicinal plants having various chemical constituents which are bioactive and could be of the therapeutic use, known as secondary metabolites. These are the organic compounds which aid them in their normal growth and development. The systematic study regarding presence of phytochemicals may aid in the analysis, pharmacological and property predication studies. The mechanistic mode of plants producing therapeutic effects can also be better understood and investigated if the given information scrutinized well. In the given study, ethanolic extracts were analyzed for quantitative phytochemical estimation of total flavonoids and phenolics. The study inferred the presence of active phytoconstituents, flavonoids and terpenoids in particular. The extracts were further studied by LC-MS. The phytochemical identification was done on the basis of m/z ratio obtained for each individual secondary metabolite. The numeral analysis for ketones present as secondary metabolite is done along with frequency of occurrence of particular ketone in other Nakshtra plants. The representative ketones of medicinal and biological importance are also identified. The given study paves a way to the systematic analysis of the phytochemicals pertaining to ketones or any other secondary metabolite under study and sheds light on identification and property predication which may help in formulating a drug and study its pharmacology.
Keywords: Nakshtra Plants, Phytochemicals, LC-MS, Flavonoids and Terpenoids, Ketones

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