S. Sasi Mounika1 ,V. Karuna Sree2
1NRI College of Pharmacy, Vijayawada, Andhra Pradesh
2 KVSR Siddhartha College of Pharmaceutical sciences, Vijayawada, Andhra Pradesh
A B S T R A C T
Aim: Herbal therapies had become integral part of health care sciences.Urolithasis is a condition in which crystals in urine combines to form stones. The study evaluates antiurolithiatic activity of different extracts of the plants Cucumis sativus, Citrullus lanatus and Tribulus terrestris. In addition to that preliminary phytochemical screening of plants extract showed the presence of several phytochemicals. Method: Invitro antiurolithiatic activity can be studied by preparing calcium oxalate crystals by homogenous precipitation method and studying the effect of plant extracts on their ability to dissolve these crystals. In the present study, undissolved calcium oxalate was estimated by Kramer and Tisdall method. A total of 8 semi permeable membranes were taken and exactly 10 mg of calcium oxalate and 100mg of various drug extracts are packed in semipermeable membrane separately and this is allowed to suspend in a flask containing 100ml of 0.1M TRIS buffer.The contents remaining in the semi permeable membrane were titrated with KMNO4 till a light pink colour end point was obtained by adding 2ml of 1N sulphuric acid. The result obtained are compared for activity with standard drug Tamsulosin hydrochloride (0.2mg). Conclusion: The study evaluates antiurolithiatic activity of different extracts of the plants along with phytochemical screening which resulted in presence of important phytochemical constituents like alkaloids, carbohydrates, fats and fixed oils, saponin glycosides, tannins, steroids and terpinoids. All aqueous, methanol, chloroform extracts of Cucumis sativus, Citrullus lanatus and Tribulus terrestris possessed significant activity in treatment of kidney stones.
Keywords: Antiurolithiatic activity, phytochemical screening, Kramer and Tisdall method