Sandeep Akkam*1, Dr. C. Madhusudhan Chetty2, D. Chinna Babu3, V. Vijay Kumar4, Y. Chaitanya Kumar5, N. Swaroop Reddy6
Department of Pharmacy Practice, Santhiram college of Pharmacy, Nandyal, Andhra Pradesh, India.
A B S T R A C T
The plant Pergularia daemia has been traditionally used for treating various diseases. The present study was aimed to investigate the anti-inflammatory activity of aqueous extract of the whole plant of Pergularia daemia by human red blood cell (HRBC) membrane stabilization method. The prevention of hypo tonicity induced human red blood cells (HRBC) membrane lysis was taken as a measure of the anti-inflammatory activity. Phytochemical assay was done to find the main active constituents. Among all the concentrations 400μg/ml showed significant anti-inflammatory activity and 78.97% protection and the standard drug diclofenac showed 86.68% protection. IC50 values were calculated and found to be 217 μg/ml and 200µg/ml for plant extract and standard diclofenac respectively. The results obtained in the present study suggest that Pergularia daemia can be a potential source of anti-inflammatory agents.
Keywords: Pergularia daemia, anti-inflammatory activity, HRBC, IC50.