Phytochemical screening and antibacterial activity of leaves of Eclipta alba (L.) Hassk on important species of Bacteria

B. Kiran¹, V. Lalitha² and K.A. Raveesha^3
1Assistant Professor, Department of Botany, Sikkim University, 6^th Mile, Tadong, Gangtok Sikkim-737102, India.
²Assistant Professor, Department of Studies in Botany and Microbiology, Maharanis Science College for Women, Bangalore-560001, Karnataka, India
³Professor, Department of Studies in Botany, Manasagangotri, University of Mysore, Mysore- 570 006, India

Abstract
In vitro evaluation of antifungal activity of aqueous extract of Eclipta alba (Leaves) were tested against four gram negative bacteria at 10µl-100 µl concentration. In E. coli maximum and significant activity was observed in 80-100 µl concentration tested and recorded 2.0mm to 34.0mm inhibition. In K. pneumonia, the percent inhibition was up to 25.0 mm inhibition. In P. aeruginosa the inhibition percentage was in the range of 1.0mm to 21.0mm respectively. In S. typhi maximum inhibition was observed and recorded 35.0mm inhibition in 100 µl concentration and significant activity was also observed in 80 and 90 µl concentration. All the result was compared to standard antibiotics Chloramphenicol (25mg) and Gentamicin (25mg) and recorded 28.0mm to 35.0mm inhibition in all the test bacteria.
Keywords: Eclipta alba, phytochemical analysis, Gram negative bacteria, antibacterial activity