Friday , 28 February 2020

Method Development and Validation for Simultaneous Estimation of Propranolol and Valsartan by using RP-HPLC in Bulk and Pharmaceutical Dosage Form.

V. Bhargavi2, P. Sowjanya3, Dr. T. Rajesh4, Dr. Gampa Vijaya Kumar*1
1Professor and Head, Dept. of Pharmacy, KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy.
2,3,4Deapartment of Pharmacy, KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy.

A B S T R A C T
A simple, sensitive and precise reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of Propranolol and Valsartan in pharmaceutical dosage form. The Chromatography was carried out on column ODS inertsil C18 (4.6mm x 250mm, 5μm) column using Methanol: Buffer (KH2PO4) in the ratio of 60:40, as the mobile phase at the flow rate of 1.0 ml/min with UV detection at 272nm. The retention times of Propranolol and Valsartan 2.955 min and 3.532 min respectively. The method was validated in terms of linearity, precision, accuracy, limit of detection, limit of quantification. The LOD for Propranolol and Valsartan is 0.25mg/ml & 0.34mg/ml and LOQ for  Propranolol and Valsartan is 0.77mg/ml & 1.05mg/ml respectively. The coefficient of variance for both the drug was more than 0.999.The proposed method can be used for determination of these drugs.
Keywords: Propranolol and Valsartan, RP-HPLC

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