Riyazunnisa D1*, Sultan Ali2, Karimulla Shaik3
1Dept. of Pharmacology, Sri Lakshmi Venkateswara Institute of Pharmaceutical Sciences, Proddatur, A.P, India -517132
2Department of Pharmacology, SAFA College of Pharmacy, Thandrapadu, Kurnool, A.P, India -518007
3Department of Pharmacology, Narayana Pharmacy College, Chintareddypalem, Nellore, A.P, India -524003
A B S T R A C T
In the present study, the eﬀect of hydroalcohoilc extract of Orthosiphon thymiflorus leaves (HAEOT) was studied on glucose induced insulin secretion in rat pancreas with an evaluation of their mechanism of action. Isolation and preparation of pancreas by collagenase digestion method and Insulin secretion from islets were observed with graduate concentrations of HAEOT. There was a concentration-dependent increase in insulin release when islets were incubated with various concentrations of HAEOT at 10 and 60 min. For inhibition of insulin from pancreas, the pancreas islets were pre incubated with diazoxide along with and without 100 mg of HAEOT. Incubation of pancreatic islets with glucose and 100mg of HAEOT in the presence of diazoxide caused a 24.76% (10 min) and 26.73 % (60 min) inhibition in insulin release. This study clearly indicated that Orthosiphon thymiflorus reverse the effect of diazoxide. It can be concluded that the insulin secretagogue effect of hydroalcoholic extract of Orthosiphon thymiflorus leaves regulates the insulin secretion of islets of pancreas because of its antioxidant property.
Keywords: Orthosiphon thymiflorus, diazoxide, Insulin secretagogue, Glucose induced insulin secretion.