Friday , 15 December 2017

In-vitro Studies of Lectin Specific Changes on Erythrocyte Membrane in Malaria

Suvarna T Jadhav*1, Ulhas S. Mali2, Nilima R. Patil3,Smita Patil4, Pooja Pharane5
1Assistant Professor, Biochemistry Department, Bharati Vidyapeeth University Dental College and Hospital, Sangli.416 414.  Maharashtra, India.
2Associate  Professor, Department of Pathology, Bharati Vidyapeeth University Dental College and Hospital, Sangli.
3Assistant Professor, Department, of Microbiology, Bharati Vidyapeeth University Dental College and Hospital, Sangli.
4Assistant Professor, Department of Physiology, Bharati Vidyapeeth University Dental College and Hospital, Sangli.
5Assistant Professor, Department of Periodontology, Bharati Vidyapeeth University Dental College and Hospital, Sangli.

A B S T R A C T
PTA lectin causes haemagglutination by acting on sialic acid ligand on the surface of RBC’s. In malarial infection, the parasite causes surface changes in RBC leading to blockage of sialic acid ligand. Resulting in to the decrease in haemaggulinations by PTA lectin methods. Purified lectin prepared from pedilanthus tithymaloides is used to determine haemagglutination in normal and malarial parasite RBC’s. The titer of lectine causing haemagglutination is observed. haemagglutination by PTA lectin in parasitized RBC’s is significantly low as compared to haemaglutination in normal RBC’s. Changes in surface ligands on RBC’s due to malarial infection changes the haemagglutination property of RBC’s by PTA lectin, with resultant decrease in haemagglitunation can have application in the diagnosis and treatment of malarial infection.
Keywords: RBC, haemagglitunation, pedilanthus tithymaloides, PTA lectin, ligand.

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