Sunday , 23 July 2017

Effect of alcohol concentration in Diffusion medium on diffusion of Terbinafine gel through artificial membranes

Chamarthi R P Kishore*, Dr. G V Krishna Mohan
Department of Chemistry, K L University, Guntur-522502, Andhra Pradesh, India.

A B S T R A C T
The aim of the present experiment was to compare the diffusion property of Terbinafinein diffusion medium containing ethanol in different concentrations. Diffusion study was conducted on artificial membranes with different pore size of 0.22µ. A simple, accurate and sensitive HPLC method was developed and validated for the estimation of Terbinafine in diffusion study. This methodology can be useful in the determination of bioequivalence between topical formulations 0.2% Triethyl amine buffer with pH adjusted to 7.5 and Acetonitrile in the ratio of 15:85 was used as a mobile phase. Flow rate was set to 1.5mL/min and detection was carried at 280nm. 100µl sample and standard solutions were injected into150x 4.6mm 2.7µ, Ascentis express C18 HPLC column. Linearity was established in the concentration range of 0.02 ppm– 3ppm.Recovery studies were done and satisfactory results were observed.
Keywords: Diffusion study, artificial membranes, gel, bio-equivalence and development.

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