Development of Rapid UV Spectrophotometric Method for the Estimation of Pioglitazone Hydrochloride in Bulk and Formulations

A. Hemanth*¹, H. Abdul Ahad^2
1V. L .College of Pharmacy, Manik Prabhu Temple Road, Raichur-584103, India.
²Balaji College of Pharmacy, Rudrampeta Bypass, Anantapur-515001, India.

A B S T R A C T
Simple, sensitive, accurate, precise and rapid ultraviolet (UV) Spectrophotometric method was developed for the estimation of Pioglitazone HCl in pure form, formulations and stability samples. For the estimation of Pioglitazone HCl, solvent system employed was acetate buffer pH 2 and wavelength of detection (λ_det) was 269nm.The developed method was used to estimate the total drug content in two commercially available oral formulations of Pioglitazone HCl and recovery studies were also carried out. Sample recovery in both the formulations using the above method was in good agreement with their respective label claims, thus suggesting the validity of the method and noninterference of formulation excipients in the estimation. The developed method was found to be stability specific and were validated as per ICH guidelines-2005, USP-2000 and statistical methods.
Keywords: Spectrophotometric determination, Methanol, acetate buffer pH 2, Pioglitazone HCl