Monday , 21 January 2019

Development and Validation of RP HPLC Method for Simultaneous Estimation of Netupitant and Palonosetron in Pharmaceutical Dosage Form

Dr. Gampa Vijay Kumar1*, B. Sravanthi2, N. Gayathri Aparna3
1Professor and Head, Dept. of Pharmacy, KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy, Telangana, India.

2KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy, Telangana,India.
3KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy, Telangana,India.

A  B  S  T  R A C T
On the basis of experimental results, the proposed method is suitable for the quantitative determination of Netupitant and Palonosetron in pharmaceutical dosage form. The method provides great sensitivity, adequate linearity and repeatability. The estimation of Netupitant and Palonosetron was done by RP-HPLC. The Phosphate buffer was pH 2.5 and the mobile phase was optimized which consists of Acetonitrile: Phosphate buffer mixed in the ratio of 80:20 % v/ v. A Symmetry C18 (4.6 x 150mm, 5mm) column used as stationary phase. The detection was carried out using UV detector at 274 nm. The solutions were chromatographed at a constant flow rate of 0.8 ml/min. the linearity range of Netupitant and Palonosetron were found to be from 25-125 mg/ml.Linear regression coefficient was not more than 0.999.The values of % RSD are less than 2% indicating accuracy and precision of the method.The percentage recovery varies from 97-102% of Netupitant and Palonosetron LOD and LOQ was found to be within limit.The proposed method is precise, simple and accurate to determine the amount of Netupitant and Palonosetron in formulation.High percentage of recovery shows that the method is free from the interference of excipients used in the formulation. So the method can be useful in the routine quality control of these drugs.

Keywords: Symmetry C18, Netupitant and Palonosetron, RP-HPLC.

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