Comparative study on the phytochemical and free radical scavenging (antioxidant) activity of ethanolic extracts from different parts of Annona squamosa L. (Annonaceae).

C. Chandrashekar, V.R. Kulkarni*
Department of Studies and Research in Chemistry, Gulbarga University, Gulbarga, Karnataka. India.

A B S T R A C T
The present study aimed at the evaluation of preliminary Phytochemical and free radical scavenging (Antioxidant) potential of extracts of different parts of Annona squamosa L. plant and their correlation with the total phenolic contents (TPCs). The free radical scavenging activity of ethanol extracts were assessed by 2, 2‑diphenyl‑1‑picrylhydrazyl assay and TPCs by Folin‑Ciocalteu method. The percentage radical scavenging activities of samples were compared to the standard butylated hydroxyl anisole (BHA) and catechol. The leaf extract shown marked quantity of phenolics and promising free radical scavenging (antioxidant) activity followed by seed and root extracts. IC₅₀ values for the scavenging activities of DPPH exhibited by the ethanol extracts of leaf, seed and root were 80μg/mL, 790 μg/mL, and 980 μg/mL, respectively. The total phenolic content of extracts of Annona squamosa. L exhibited the leaf (0.114mg/g), seed (0.091 mg/g) and root (0.022mg/g).The reductive ability of the extracts was found to increase in a concentration dependent manner. The results suggested that the phenolic compounds in extracts are most likely to be responsible for the observed free radical scavenging antioxidant activity.
Keywords: Annona squamosa L, custard apple, antioxidant activity, 2, 2‑diphenyl‑1‑picrylhydrazyl assay, total phenolic content, phytochemicals.