Sunday , 28 May 2017

Comparative Analysis of Antioxidant and Antimicrobial Activity of Grape Seed and Grape Skin

Ms. Sneha V & Dr. V. Judia Harriet Sumathy
Postgraduate & Research Department of Biotechnology, Women’s Christian College, Chennai – 600 006.

A B S T R A C T
Grape (Vitis vinifera) skin and seed are considered to be rich sources of poly-phenolic compounds, mainly monomeric catechin and epicatechin, gallic acid, polymeric and oligomeric procyanidins. Resveratrol is found in widely varying amounts among grape varieties, primarily in their skins and seeds, which, in muscadine grapes, have about one hundred times higher concentration than pulp. Fresh grape skin contains about 50 to 100 micrograms of resveratrol per gram. These grape seed and skin extract compounds act as anti-mutagenic and antiviral agents. Beneficial aspects include inhibition of carrageen in or dextran-induced edema which stabilizes the capillary wall and improvement of visual performance in humans. The present study is aimed at extracting seed and skin of red grapes with methanol and perform the Phytochemical tests for both using qualitative analysis. Furthermore Total Phenolic content by Quantitative analysis, Column Chromatography by GC-MS to confirm the secondary metabolites for skin and seed, Antioxidant activity by DPPH, Hydrogen Peroxide and Total antioxidant capacity and Antibacterial activity for grape skin and seed extract is also assessed and compared for its efficiency.
Keywords: Grape skin and seed, Resveratrol, Procyanidins, Antioxidant and Antimicrobial Property.

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