Biochemical Study of Exopolysaccharide from Klebsiella Spp. isolated from the Gut of Poecilia Sphenops

Ms. Meghna Sudhesh & Dr. V. Judia Harriet Sumathy
Postgraduate & Research Department of Biotechnology, Women’s Christian College, Chennai – 600 006.

A B S T R A C T
Exo-polysaccharides (EPS) are environment friendly natural polymers secreted by microorganisms to the surrounding medium. Due to the presence of unique structural composition, EPS shows diverse applications such as in pharmaceutical, and cement based construction industry, etc. Microbial derived EPS have advantages over traditionally used polysaccharides from plant source and seaweed-derived gums as the later are easily affected by environmental factors. EPS show different properties like, thickening, gelling, emulsifying, and ability for immobilisation. Bacteria offers wide diversity of exo-cellular substances with characteristic composition and properties, that provides opportunities for the development of new commercial product with improved properties. The present study is aimed at isolating EPS from the gut of Poecilia sphenops and conduct biochemical analysis. The extra cellular polysaccharide polymer produced from Klebsiella spp. can find its application for emulsification at the industrial level.
Keywords: EPS, Klebsiella spp, Poecilia sphenops, Biochemical Study and Emulsification