Ratmawati Malaka*, Efendi Abustam and Sudirman Baco
Hasanuddin University, Faculty of Animal Science, Jl. Perintis Kemerdekaan Km 10 Tamalanrea, Makassar, Indonesia.
A B S T R A C T
An extracellular polysaccharide (EPS) was purified by ethanol precipitation from culture supernatant of Ropy strains of Lactobacillus delbrueckii ssp. bulgaricus, a lactic acid bacteria that was isolated from commercial yoghurt. The EPS was produced under aerobic conditions in a medium Skim Milk Reconstitution (SMR) 10% (w/v). This EPS have studied to potential health – promoting effect as antitumor activity. The objective of this study was to determine of potential of the EPS on proliferation of tumor cell in vitro. EPS with different concentration (0.01; 0.02; 0.05; and 0.001 mg/ml) was examined in vitro for antitumor activity on Leukemia K-562 and Hela cell. Result of these studies had showed that EPS in doses 50 mg/ml can inhibited of 45.4 % of tumor leukemia and 59.2% for tumor human cervix (Hela cell).
Keywords: antitumor activity, Lactobacillus bulgaricus, antiproliferation, exopolysaccharide (EPS).