Antiproliferative and Antioxidant activities of Picea smithiana (Wall) Boiss oil

About author
Wajaht A Shah*and Mohd Yousuf Dar
Department of Chemistry, University of Kashmir, Hazratbal, Srinagar, J & K-190006, India.

Abstract
Identificaton and investigation of Picea smithiana (Wall) Boiss oil for its antiproliferative and antioxidant activities. Picea smithiana (Wall) Boiss oil was extracted from aerial parts of the plant material by hydrodistillation method (0.06%). The oil was then subjected to GC-MS for identification of oil constituents. Oil (12.5, 25, 50, 100µg/ml) was also evaluated for its antiproliferative activity by in-vitro MTT {3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide} assay against a panel of human cancer cell lines like human monocyte (THP-1), lung carcinoma (A-549), liver adenocarcinoma (HEP-1) and ovarian carcinoma (IGR-OV-1), while antioxidant activity was evaluated by in-vitro DPPH assay method. GC-MS of Picea smithiana oil indicated the presences 36-constituents were identified by mass spectroscopy which was the first time report from this plant, with delta-3-carene (26.49%), limonene (25.88%), and beta pinene (14.91%), α-pinene (6.62%), Camphene (4.84%), Alpha-terpinolene (2.48%), P-cymene (1.40%) and beta phelledrene (0.98%) as major constituents. Picea smithiana oil (12.5, 25, 50, 100µg/ml) showed significant antiproliferative activity in MTT assay against a panel of human cancer cell lines like human monocyte (THP-1), lung carcinoma (A-549), liver adenocarcinoma (HEP-1) and ovarian carcinoma (IGR-OV-1). The antioxidant activity was also found in the oil in significant manner. Picea smithiana oil was first time evaluated for its constituents and we found that it contains 32 constituents; some of these were in major quantity. Picea smithiana oil showed the antiproliferative activity in human monocyte (THP-1), lung carcinoma (A-549), liver adenocarcinoma (HEP-1) and ovarian carcinoma (IGR-OV-1) which may lead to treat lung, liver, ovarian and other cancers. Antioxidant activity also supports its antiproliferative action.
Keywords: Picea smithiana oil, Antiproliferative activity, Antioxidant activity, Gas-chromatography with Mass spectroscopy