Friday , 23 August 2019

Antioxidant, Hepatoprotective Activity and RP-HPLC Analysis of Moringa Oleifera Pods Methanolic Extract against Alcohol Induced Hepatotoxicity

Konda Ravi Kumar, A. Prathyusha*
Department of Pharmaceutical Sciences, Acharya Nagarjuna University, Guntur, Andhra Pradesh.

A  B  S  T  R A C T
In this study the pods of Moringa oleifera were extracted with methanol and subjected to analysis of the polyphonolic compounds by RP-HPLC method and to appraise the free radical scavenging activity and hepatoprotective activity aligned with the injury caused by alcohol to liver in Wister albino rats. Polyphonolic compounds like quercetin, gallic acid and rutin were seen in RP- HPLC study. The extracted Moringa Oleifera pods in methanol and ascorbic acid (standard) was subjected to in vitro free radical protective activity by inhibition of lipid peroxide, superoxide, hydroxyl and DPPH (2,2-diphenyl-1-picryl hydrazyl) radical. The in vivo antioxidant and hepatoprotective effect of Moringa oleifera pods which was methanolic extract was investigated at selected doses of 100, 200 and 400 mg/kg body weight per day, and standard drug Silymarin at dose of 100 mg/kg body weight per day were administered for 25 days by oral route. The ethanol (40%) used as a hepatotoxicant and dose selected for liver injury was 3.76 gr/kg body weight per day given orally for 25 days. The IC50 values of methanolic extract of Moringa Oleifera pods was found to be 256.50µg/ml for super oxide radical scavenging activity, 287.50μg/ml for DPPH radical scavenging activity, 363.30µg/ml for hydroxyl radical scavenging activity and 805.41µg/ml for inhibition of lipid peroxidation. Ethanol treatment in rats caused severe liver damage indicated by marked elevated liver biomarkers like serum aspartate aminotransferase (SGPT), alanine aminotransferase (SGOT), alkaline phosphatase (ALP), total bilirubin and physical parameters like liver weight and liver volume, as well as pronounced decreased in serum total protein compared to normal rats. Oxidative stress status was appeared on alcohol control evident as recorded significant dwindling in levels of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT), concurrence with remarkable amplification on oxidative stress marker; malondyaldehyde (MDA) concentrations with respect to normal values. The Moringa oleifera pods which was extracted in methanol and standard Silymarin on oral administration significantly protect the liver from toxic dose of ethanol on the above serum liver biomarkers, antioxidant and physical parameters in curative model. All these palpable results were confirmed by histopathological observations, which indicated that the presence of polyphonolic compounds in methanolic extract of Moringa oleifera pods showed normalization of degenerated and fibrotic liver tissue as of alcohol treated group.
Keywords: Moringa oleifera pods, RP-HPLC, Antioxidant, Alcohol, Hepatoprotective.

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