D. Yashwanth Kumar*1, D.S.S.N. Neelima1, Pradeep Kumar Choda2, Namani Srilatha2
1Scientific and Applied Research Center, Hyderabad, Telangana, India
2Department of Pharmacology, Teegala Krishna Reddy College of Pharmacy, Hyderabad, Telangana, India
A B S T R A C T
Acute inflammation is a short-term process, usually appearing within a few minutes or hours and ceasing upon the removal of the injurious stimulus. It is characterized by five cardinal signs [Dolor (pain), Calor (heat), Rubor (redness), Tumor (swelling) and Functio laesa (loss of function]. Symphytum officinale Linn belongs to the family boraginaceae. It is a herb commonly known as Comfrey. Many reports have demonstrated the usefulness of the extracts of this plant in many diseases and conditions like lung disorders, gastritis, and stomach ulcer and bleeding. The present study was carried out to assess the possible anti-inflammatory effect of methanolic extract of roots of Symphytum officinale Linn (MERSO) using egg white-induced edema in rats (acute model of inflammation). The anti-inflammatory effect of MERSO was evaluated in acute inflammation model using 30 Wistar albino rats and divided into five groups including normal saline 10 ml/kg orally, diclofenac 5 mg/kg IP and MERSO 250,500 & 700mg/kg b.wt orally. Thirty minutes post treatment, inflammation was induced by injecting 0.1 ml of fresh egg albumin into the sub plantar surface of the right hind paw and mean increase in paw edema was measured 60 min, 120 min, 180 and 240 min after induction of inflammation using plethysmometer. Suppression of paw inflammation by either diclofenac or MERSO and the percentage of inhibition of paw edema were assessed. The data obtained from this study reported that orla administration of extract significantly (P<0.05, P<0.01) inhibited raw egg albumin- induced rat paw oedema as compared to control group. Maximum inhibitory effect (33.53%) was observed at a dose of 750mg/kg, at the end of 240 minutes when compared to control group
Keywords: Acute inflammation; Symphytum officinale Linn; Egg white-induced edema.