Anti-inflammatory activity of aqueous extract of Mimusops elengi in human whole blood and peripheral blood mononuclear cells

Amit Gupta*, Pallavi R Khamkar and Sushama R Chaphalkar 
Department of Immunology, Vidya Pratishthan’s, School of Biotechnology (VSBT), Baramati, District Pune, Maharashtra, India

Abstract
The aim of the present study is to present a simple, fast and reliable method of anti-inflammatory activity of medicinal plants especially Mimusops elengi in human whole blood and peripheral blood mononuclear cells using flow cytometry. To achieve this objective, aqueous extract isolated from the different parts of the plants especially leaf, stem and root and checked its anti-inflammatory activity in human whole blood and peripheral blood mononuclear cells (PBMC). Human whole blood and PBMC were pre-incubated with different concentration of aqueous extract of Mimusops elengi  to determine the blood counts, estimation of monocyte marker CD14 and also estimate the Th1(TNF alpha) cytokine profile of Mimusops elengi from peripheral blood mononuclear cells using lipopolysaccharide. Mimusops elengi showed dose-dependently and significantly reduced a range of blood counts especially monocytes and its surface marker CD14 and also suppressed the pro-inflammatory cytokine i.e. tumor necrosis factor in peripheral blood mononuclear cells. The presented data indicate that Mimusops elengi has broad anti-inflammatory effects in human whole blood and PBMC.
Keywords: Mimusops elengi, anti-inflammatory, lipopolysaccharide, monocyte