Parsa Sivarama Krishna*, G. Devika Subramaniyan
Bomma Institute of Pharmacy, Allipuram, Khammam, Telangana
A B S T R A C T
A simple, accurate, precise method was developed for the simultaneous estimation of the Raltegravir and Lamivudine in Tablet dosage form. Chromatogram was run through Inertsil ODS 3V(250×4.6mm)5µm column by using a mixture of Phosphate buffer and acetonitrile in a ratio of 55:45 v/v was pumped through column at a flow rate of 1.0ml/min and detection wavelength was at 275nm. The %RSD of the Raltegravir and Lamivudine were and found to be 1.14 and 1.19 respectively. The % recovery was obtained as 100.68 and 102.33for Raltegravir and Lamivudine respectively. LOD, LOQ values are obtained from regression equations of Raltegravir and Lamivudine were 0.21, 2.4µg/ml and 0.6, 4.2µg/ml respectively. The developed method is applicable for routine quality control analysis of selected combined dosage forms.
Keywords: Raltegravir, Lamivudine, RP-HPLC