Jyothi Keerthi*, G. Devika Subramaniyan, Ramesh Petchi, Rajendran
Bomma Institute of Pharmacy, Allipuram, Khammam, Telangana, India
A B S T R A C T
A Reverse Phase High Performance Liquid Chromatography method was developed for the determination of Enalapril and Hydrochlorothiazide in bulk and Pharmaceutical dosage form. The separation was achieved on an Inertsil ODS (250×4.6mm),5µm column by using a mixture of Phosphate buffer and acetonitrile in a ratio of55:45 v/v at a flow rate of 1ml/min. The detection was made at 215 nm. Calibration curve was linear over the concentration range of 10-60 μ g/ml of Enalapril and Hydrochlorothiazide. The proposed method was validated as per the ICH guidelines. The method was rapid, accurate, precise, specific and found to be suitable for the quantitative analysis of the drug in Bulk and Pharmaceutical dosage form.
Keywords: Enalapril, Hydrochlorothiazide, RP-HPLC