Friday , 23 June 2017

Analytical Method Development and Validation for the Simultaneous Estimation of Beclomethasone Dipropionate and Clotrimazole by RP-HPLC Method in Bulk and Pharmaceutical Dosage Form

V. Padmaja*1, Dr. Y. Padmavathi2
1Dept. of Pharmaceutical Chemistry, G. Pullareddy College of Pharmacy, Hyderabad, Telangana, India
2Professor, G. Pullareddy College of Pharmacy, Hyderabad, Telangana, India

A B S T R A C T
The chromatographic conditions were successfully developed for the separation of Beclomethasone dipropionate and Clotrimazole by using column C18 [Macherey-Nagel Nucleosil], 4.6x250mm, 5 microns flow rate was 1.2 ml/min, mobile phase ratio was Phosphate buffer: Methanol (30:70 v/v), detection wavelength was 254 nm. The Spectroscopic method was done in solvent using methanol and the instrument used was DIONEX HPLC system. UV D340U detector. The retention times for Beclomethasone dipropionate and Clotrimazole were found to be 9.5 min and 14.5 min. The analytical method was validated according to ICH guidelines (ICH, Q2 (R1)). The linearity study of Bclomethasone dipropionate and Clotrimazole was found in the concentration range 6.25-18.75 mcg/ml and 250-750 mcg/ml and correlation coefficient (r2) was found to be 0.999 and 0.999 respectively, % recovery was found to be 99.44 to 100.20 and 99.75 to 100.05% respectively. %RSD for repeatability and precision was found to be <2. LOD values for Beclomethasone dipropionate and Clotrimazole were found to be within the limits and LOQ values for Beclomethasone dipropionate, Clotrimazole were found to be within the limits respectively.   .
Keywords: Beclomethasone dipropionate, Clotrimazole, HPLC

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