Tuesday , 27 June 2017

Analytical Method Development and Validation for the Simultaneous Estimation of Amlodipine and Perindopril by RP-HPLC Method in Bulk and Pharmaceutical Dosage Form

Ramesh Dhani, J. Sudha Rani*, M. Gobinath, V. Haribaskar
Department of Pharmaceutical Analysis, Ratnam Institute of Pharmacy, Pidathapolur, Nellore, Andhra Pradesh, India

A B S T R A C T
The chromatographic conditions were successfully developed for the separation of Amlodipine and Perindopril  by using Kromosil C18 Column (250mm x 4.6mm)5µm, flow rate was 1ml/min, mobile phase ratio was Methanol: Phosphate buffer PH 3 (35:65 v/v), detection wavelength was 254 nm. The Spectroscopic method was done in solvent using methanol and the instrument used was Shimadzu UFLC-20 AD Chromatographic system (japan) . SPD-M20A diode array detector LC 20 software. The retention times for Amlodipine and Perindopril were found to be 2.589 min and 3.7111 min. The analytical method was validated according to ICH guidelines (ICH, Q2 (R1)). The linearity study of Amlodipine and Perindopril was found in the concentration range 20 ppm-60 ppm and 10ppm -30 ppm and correlation coefficient (r2) was found to be 0.999 and 0.999 respectively, % recovery was found to be 95.0% and 105.0% respectively. %RSD for repeatability and precision was found to be <2. LOD values for Amlodipine, Perindopril were found to be 0.001 and 0.005 respectively and LOQ values for Amlodipine, Perindopril were found to be 0.004 and 0.015 respectively.   .
Keywords: Amlodipine, Perindopril, HPLC

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