Analytical Method Development and Validation for the Estimation of Lacidipine in Bulk and Dosage Form by RP-HPLC

Dandu Girija^1*, Akkimi Padma¹, A. Thirupathireddy², B. Venkateswara reddy^3
1Assistant professor, Department of Pharmaceutical Analysis, Sankar Reddy Institute of Pharmaceutical sciences, Salakalveedu(v), Bestavaripeta(M), Prakasam(Dist) Andha pradesh       pin -523370.
²Associate Professor, Department of Pharmaceutics, Sankar Reddy Institute of Pharmaceutical sciences, Salakalveedu(v), Bestavaripeta(M), Prakasam(Dist) Andha pradesh  pin – 523370.
³Professor, Department of Pharmaceutics, Sankar Reddy Institute of Pharmaceutical sciences, Salakalveedu(v), Bestavaripeta (M), Prakasam(Dist) Andha pradesh  pin – 523370.

A B S T R A C T
The aim of present research work method development and validation for the quantitative estimation of Lacidipine in pure and Pharmaceutical dosage forms by RP-HPLC. Chromatographic separation was carried out on an Intersil C-18 column using a mobile phase consisting of Phosphate Buffer pH-3:Methanol (60:40 v/v). The mobile phase was pumped at a rate of 1.0 ml/min and the UV detection wavelength was measured at 284 nm. The linearity was found to be in the range of 50-120 μg/ml and retention time was 4.7 min. Mean percentage recovery of Lacidipine was found to be 99.89%.  The developed method was validated stastically according to ICH guidelines(Q2R1). So the developed method was linear, precise, accurate and robust, the proposed method wassuccessfully applied for the quantitative determination of Lacidipine in pharmaceutical dosage forms.
Keywords: Lacidipine, RP-HPLC, Method validation, Intersil C-18 column, Retention time, Mobilephase