Sunday , 9 August 2020

Analytical Method Development and Validation for the Estimation of Brivaracetam in Bulk and Its Dosage Form by RP-HPLC

Patta Salomi*, S. Rama Thulasi, K. Ravindra Reddy
P. Rami Reddy Memorial College of Pharmacy, Kadapa-516003, Andhra Pradesh, India

A simple reverse phase liquid Chromatographic method has been developed and subsequently validated for determination of Brivaracetam. The mobile composition of Potassium Dihydrogen orthophosphate (0.02M): Methanol 40:60v/v Buffer pH 6.0 adjusted with ortho phosphoric acid, waters column of C18 (250X4.6 ID) 5μm and flow rate 1.2 ml/min, using UV detection at 290 nm. run time 4.0 min efficient and reproducible method was developed for determination of Brivaracetam in tablet dosage form. The retention time of Brivaracetam were found to be 2.35 min and Results of analysis were validated statistically and by recovery studies. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Brivaracetam bulk drug and in its pharmaceutical dosage form.
Keywords: Brivaracetam, Development, Forced degradation, HPLC, Validation

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