Wednesday , 23 August 2017

Analytical Method Development and Validation for Simultaneous Estimation of Metformine Hydrochloride and Pioglitazone by Using UV Spectrophotometry

N. Geetha*, D. Sireesha, Dr. Vasudha bakshi
Department of Pharmaceutical Analysis and Quality assurance, Anurag group of institutions (Formerly Lalitha college of pharmacy), Hyderabad, Telangana, India

A B S T R A C T
A simple, Accurate, precise method was developed for the simultaneous estimation of the Metformine hydrochloride and Pioglitazone in Tablet dosage form. Chromatogram was run through C18, 4.6 mm, 5mm Make:x Terra Mobile phase containing Acetonitrile and Water in the ratio of 50:50 was pumped through column at a flow rate of 1ml/min. Optimized wavelength for Metformin   and Pioglitazone was 242nm. %RSD of the Metformin   and Pioglitazone were and found to be less than 2. Lnearity range of Metformin and Pioglitazone 25-125mg/ml. Correlation coefficient   is 0.999.
Keywords: Metformin, Pioglitazone, UV-Spectrophotometry

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