Thursday , 29 June 2017

Analytical Method Development and Validation for Simultaneous Estimation of Cilnidipine and Telmisartan in Combined Tablet Dosage Form by Using RP-HPLC

R. Chandra Sekhar*, Prof. Ravindernath
Post graduate student, University College of Technology, Osmania University, Hyderabad, Telangana, India.

A B S T R A C T
Objective: To develop and validate a suitable method for the simultaneous estimation of Cilnidipine and Telmisartan in Tablet dosage form by using RP-HPLC method. Methods: The chromatographic separation was performed on ZORBAX SB-PHENYL, 250X 4.6mm. The mobile phase was prepared by mixing of Orthophosphoric acid and methanol in the ratio of (80:20%) v/v that run isocratically at the flow rate of 1.2ml/min. and detection of all the eluents carried out by PDA Detector. 
Results: The Rt of Cilnidipine and Telmisartan were found to be 3.748 min and 2.083 min. Percentage recoveries of Cilnidipine and Telmisartan were obtained in the range of 99.9% and 99.6% respectively. The limit of detection of Cilnidipine and Telmisartan is 3.713mg/ml and 2.080mg/ml respectively. The limit of Quantification of Cilnidipine and Telmisartan is 3.713mg/ml and 2.081mg/ml respectively. Conclusion: A new sensitive, simple, and stability indicating high performance liquid chromatographic (HPLC) method has been developed and validated for determination of Cilnidipine and Telmisartan.
Keywords: Cilnidipine and Telmisartan, RP-HPLC, PDA Detector, UV.  

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