Monday , 18 February 2019

Analytical Method Development and Validation for Ivermectin and Albendazole in Combine Dosage Form by RP-HPLC

Dr. Gampa Vijay Kumar 1*, B. Sravanthi 2, A. Praveen3
1Professor and Head, Dept. of Pharmacy, KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy, Telangana, India.
2,3KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy, Telangana, India.

A B S T R A C T
New method was established for simultaneous estimation of Ivermectin and Albendazole by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Ivermectin and Albendazole by using ACE C18 column (4.6×150mm) 5µ, flow rate was 1.2 ml/min, mobile phase ratio was (70:30 v/v) methanol: Phosphate buffer pH 3 (pH was adjusted with orthophosphoricacid), detection wavelength was 240nm. The instrument used was Shimadzu, model No. SPD-20MA LC+20AD, Software- LC-20 Solution. The retention times were found to be 2.344 mins and 3.284 mins. The % purity of Ivermectin and Albendazole was found to be 101.27% and 99.97% respectively. The system suitability parameters for Ivermectin and Albendazole such as theoretical plates and tailing factor were found to be 4668, 1.3 and 6089 and 1.2, the resolution was found to be 6.0. The analytical method was validated according to ICH guidelines (ICH, Q2 (R1)). The linearity study n Ivermectin and Albendazole was found in concentration range of 50µg-250µg and 5µg-50µg and correlation coefficient (r2) was found to be 0.999 and 0.999, % recovery was found to be 99.56% and 99.48%, %RSD for repeatability was 0.2 and 0.2, % RSD for intermediate precision was 0.2 and 0.1respectively. The precision study was precise, robust, and repeatable.LOD value was 3.17 and 5.68, and LOQ value was 0.0172 and 0.2125 respectively. Hence the suggested RP-HPLC method can be used for routine analysis of Ivermectin and Albendazole in API and Pharmaceutical dosage form.
Keywords: ACE C18 column, Ivermectin and Albendazole, RP-HPLC

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