Monday , 29 May 2017

Analytical Method Development and Validation for Apixaban by RP-HPLC

Md. Abdul Majeed*1, Dr.K.Vijaya1, B. Anitha2
1Department of Pharmaceutical Analysis, Monad Univeristys, Hapur, Rajasthan, India
2SARC (Scientific and Applied Research Center), Hyderabad, Telangana, India

A B S T R A C T
A simple and precise RP-HPLC method was developed for the determination of Apixaban dosage form the chromatographic separation was achieved on an Zorbax Bonus RP (250 X 4.6mm) column with a mobile phase contain the gradient mixture of the solvents (Acetonitrile: water) in the ratio of 90:10(v/v).Column temperature is 400C. The eluted compounds were monitored at 270nm and the run time was 18mints.This method was validated for accuracy, precision, linearity, and robustness as per ICH guidelines. The development and validated RP-HPLC method is applied for identification of eluted.  
Keywords: Apixaban, RP-HPLC, Method development, Validation.

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