Friday , 28 February 2020

Analytical Method Development and Validation by RP-HPLC for Simultaneous Estimation of Metformin Hcl and Vidagliptine in Combined Tablet Dosage Form

V. Geetha2, P. Sowjanya3, Dr. T. Rajesh4, Dr. Gampa Vijaya Kumar*1
1Professor and Head, Dept. of Pharmacy, KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy.
2,3,4Deapartment of Pharmacy, KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy.

A B S T R A C T
A simple reverse phase liquid chromatographic method has been developed and subsequently validated for simultaneous estimation of vildagliptin and metformin in combination. The Chromatography was carried out on column inertsil ODS C18(4.6mm x 250mm, 5μm) column using Methanol & water in the ratio of 60:40 as the mobile phase at the flow rate of 1.0 ml/min with UV detection at 263nm. The retention time of metformin & vildagliptin is 2.869mins & 3.942mins respectively. The method was validated for linearity, accuracy, precision, specificity, limt of detection, limt of quantification and robustness. The LOD for metformin & vildagliptin is 0.33mg/ml & 0.32mg/ml and LOQ for metformin & vildagliptin is 1.01 mg/ml and 0.98 mg/ml respectively. And percentage   recovery of vildagliptin and metfomin Hcl was found to be 99.9% & 99.8%. The proposed method can be used for determination of these drugs in combined dosage forms.
Keywords: Reverse phase High performance liquid chromatography, vildagliptin, metformin Hcl.

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