Department of Microbiology, CSI Ewarts Women’s Christian College, Melrosapuram, Kancheepuram. Tamilnadu, India
A B S T R A C T
Alkalothermophilic amylase producing Bacillus Stearothermophilus from water sample.(velar estuary).The growth kinetics of the strain was optimized by using different substrate concentration with different pH and different temperature. With the study on starch utilization, the maximum growth rate was obtained at 1.5% .Starch was used as a carbon source .The maximum growth rate was obtained at pH 11 & the temperature at 50 ⁰C. The growth and substrate utilization rate in the fermentor was marginally higher than compared to those in the shake flask. In fermentor OD of Bacillus Stearothermophilus was found to be 2.986,biomas was 0.088g and substrate utilization was 1.27g at 24 hours time. The maximum amylase activity was obtained at pH 10 and at 100 ⁰ C and it was estimated as 1635.48 U/ml. The maximum enzyme activity was observed with Ca 2+,Hg 2+,Fe 2+ and the respective activity was found to be 2267,7 u/ml,2338.7 u/ml,2177,4 u/ml. Thus the present study revealed that this alkhapolic & thermophile is highly suitable for industrial application especially in detergent & starch based food industries.
Keywords: Bacillus stearothermophilus, Growth kinetics, Alkalophilic, Thermophilic Enzyme activity, Metal ions.