Shaik Mohammed Yusuf*, E. Vijay Kumar, C. Surya Svarny, E. Divya
Department of Pharmaceutical Analysis, Srinivasa Institute of Pharmaceutical sciences, Proddatur, YSR Kadapa, A.P. India
A B S T R A C T
A simple reverse phase liquid chromatographic method has been developed and subsequently validated for simultaneous determination of Ofloxacin and Ornidazole in combination. The separation was carried out using a mobile phase consisting of Ammonium acetate buffer (pH 3.0 adjusted with ortho phosphoric acid): Acetonitrile in the gradient mode. The column used was Intersil ODS-3V (250 mm x 4.6 mm i.d, 5mm) with flow rate of 1 ml / min using PDA detection at 254 nm. The retention times of Ofloxacin and Ornidazole were found to be 4.292 and 8.921 min respectively. The method was validated in the terms of its linearity, accuracy, precision, robustness, ruggedness, LOD and LOQ. The total eluting time for the both components is less than eight minutes. Proposed method was found to be simple, precise, and accurate and can be successfully applied for routine quality control analysis and simultaneous determination of Ofloxacin and Ornidazole in combined pharmaceutical drug formulations.
Keywords: Ofloxacin, Ornidazole, combination, Ammonium acetate buffer, Acetonitrile