1Punia Gudia*, 2Tandan Neeraj, 2Yadav Ashwani, 3Prasad CS
1Department of Microbiology, Shri Venkateshwara University, Gajraula, India
2Scientific and Applied Research Centre, Meerut, India
3Registrar, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, India
A B S T R A C T
The production of locally isolated (Meerut Isolate) Entomopathogenic fungi in suitable media for large scale application has not yet been studied, therefore, the present study was undertaken to evaluate grains, pulses, oilseeds and liquid media such as Potato Dextrose Broth and Sabouraud’s Dextrose Broth for the mass production of B. bassiana. Studies revealed that dry weight of Meerut (UP) isolate varied from 0.623 g to 0.811 g on different media. Growth on broth media indicated maximum dry matter production in chickpea (0.811 g) followed by SDB (0.802 g) > pea (0.796) > soybean (0.751 g) > cowpea (0.715 g) > PDB (0.711) > urd (0.663 g) > lentil (0.623 g) and groundnut (0.582 g) .Significant differences in conidial count were observed on maize, paddy, sorghum, rice and chickpea. Whereas, no significant difference was observed among rice, lentil, pea and soybean. And highest conidial count (8.97 × 107 conidia ml-1) was observed on cowpea broth followed by soybean (8.00 × 107) and pea (8.00 × 107) > Rice (7.7 × 107) > lentil (7.6× 107) = Groundnut (7.6× 107) > SDB (7.3 × 107) > urd (6.8× 107) and chickpea broth (6.2 × 107).
Keywords: Entomopathogenic Fungi , B.bassiana, Mass Production